Table 2 Median TEWL values recorded (g⋅m− 2⋅h− 1) for each step (a-d) of the skin preparation procedure including SLS treatment at different concentrations. The TEWL was measured four times at different locations on each skin piece immediately after each step of the skin preparation procedure (a-b) and three times 30 min after thawing the skin (c) and 20 min after removing the SLS treatment (d). The range of these repeated measurements, somewhat indicative of intra- and inter individual variations, is represented by min and max values
Piglet identification | PIG 1 | PIG 2 | PIG 3 | PIG 1 & 4 | PIG 2 & 4 | ||
|---|---|---|---|---|---|---|---|
Median (min; max) | Median (min; max) | Median (min; max) | Median (min; max) | Median (min; max) | |||
Preparation of skin for experiment | |||||||
a) | After rinsing with lukewarm water in situ | 11.35 (5.8; 15) | 8.10 (6.8; 12.2) | 4.25 (3.1; 6.1) | 4.85 (2.9; 15.0) | 5.35 (2.9; 9.0) | |
b) | Excised skin | 4.55 (4.3; 5.3) | 9.20 (7.9; 9.8) | 4.10 (3.8; 4.4) | 5.15 (4.3; 7.4) | 7.65 (5.0; 9.8) | |
Skin stored in freezer for up to 3 months | |||||||
c) | After thawing | 7.15 (6.6; 7.8) | 8.75 (6.8; 10.7) | 6.95 (6.2; 8.5) | 7.40 (5.5; 9.4) | 6.90 (6.0; 10.8) | |
SLS concentration | 0.5%* | 1%* | 2%* | 5%** | 10%** | ||
d) | After SLS treatment | 11.20 (7.8; 14.8) | 10.75 (9.4; 13.4) | 9.15 (7.7; 10.8) | 13.10 (9.3; 31.0) | 11.10 (9.4; 26.2) | |
∆TEWL (d-c) | 4.05 | 2.00 | 2.20 | 5.70*** | 4.20 | ||